The Hyalella azteca Bioconcentration Test (HYBIT) provides a non-vertebrate test for bioconcentration in aquatic environments. The test consists of two phases: the exposure (uptake) and post-exposure (depuration) phases. During the uptake phase, a group of H. azteca is exposed to the test...

This Test Guideline describes a standardised method to examine the transformation of chemicals in pig and cattle liquid manure under anaerobic conditions. The experiments are performed to determine the rate of transformation of the test chemical, the identity and rates of formation and decline...

The Test Guideline (TG) describes the use of cryopreserved rainbow trout (Oncorhynchus mykiss) hepatocytes (RT-HEP) as a metabolising system to determine the clearance (CL, IN VITRO, INT) of a test chemical using a substrate depletion approach. Introduction of the test chemical to the RT-HEP...

The Test Guideline (TG) describes the use of liver S9 sub-cellular fraction (RT-S9) of rainbow trout (Oncorhynchus mykiss) as a metabolising system to determine the clearance (CL, IN VITRO, INT ) of a test chemical using a substrate depletion approach. Introduction of the test chemical to the...

This test guideline describes a test procedure to gain information on dispersion stability of manufactured nanomaterials in simulated environmental media. The main purpose of this guideline is to assess the ability of a nanomaterial to attain a colloidal dispersion and to conserve this...

This Test Guideline is a screening method for the evaluation of ready biodegradability of chemicals. The test substance, normally at 20 mg C/L, as the sole source of carbon and energy, is incubated (during 28 days normally) in sealed bottles with aerobic condition containing a buffer-mineral...

This Test Guideline describes a procedure for characterising the bioconcentration potential of substances in fish, using an aqueous (standard and minimised tests) or dietary exposure, under flow-through conditions (but semi-static regimes are permissible). Independent of the chosen exposure...

This Test Guideline describes procedures designed to assess bioaccumulation of chemicals in soil oligochaetes. The parameters which characterise the bioaccumulation of a substance include the bioaccumulation factor (BAF), the uptake rate constant (ks) and the elimination rate constant (ke). The...

This Test Guideline describes the modified MITI test (II). This test permits the measurement of the Biochemical Oxygen Demand (BOD) and the analysis of residual chemicals in order to evaluate the inherent biodegradability of chemical substances which have been found by the Standard MITI Method...