showed good performance in the estimation of the optimal cut-point of a biomarker. However, when methods do not lead to the … same optimal cut-point, scientists should focus on which one is truly what they want to estimate, and use it in practice …

classifying a patient into the non-diseased or diseased group, an optimal cut-point of a continuous biomarker is desirable. Youden … measure of overall diagnostic accuracy and can be used in choosing an optimal cut-point. The proposed approach is to make use … of a generalized approach to estimate the confidence intervals of the Youden index and its corresponding optimal cut-point …