Dual-Site Fluorescent Sensor for Tracking Lysosomal Atp and H2s During Liver Injury

The liver is a vital organ of the body, and involved in many important functions. Liver injury could cause liver disease and was a serious threat to human health. During liver injury, visual evidence for changes of lysosomal degradation function is still lacking in hepatic cells. Here, we rationally designed a dual-site fluorescent sensor RDNA-1 for tracking adenosine triphosphate (ATP) and hydrogen sulfide (H 2 S) in lysosomes with Rhodamine 6G and 1,8-naphthalimide hybrid fluorophore. RDNA-1 displayed great selectivity, high sensitivity and fast response time towards ATP and H 2 S in vitro. Additionally, cell imaging results showed that RDNA-1 can be specifically localized in lysosomes for tracking ATP and H 2 S. RDNA-1 was employed to detect the changes of ATP and H 2 S levels during liver injury in real time. Meanwhile, we explored in depth the function of H 2 S as a potential drug for the repair of liver-injured cells. And importantly, RDNA-1 also successfully enabled in vivo imaging of zebrafish. Therefore, RDNA-1 provided a new approach to study the physiological mechanisms of liver injury, which is beneficial to early diagnosis and treatment of liver-related diseases. We believe that the multifunctional sensor can also be applied for the tracking of lysosomal ATP and H 2 S in other disease processes