Fish is one of the main consumable foods in Bangladesh. Fish are found naturally in river, beel, haor, pond, and baor. It is also cultivated commercially in many ways including Biofloc culture system. In this study two types of Biofloc cultivated fishes (n=10), water (n=6), and commercial fish-feeds (n=5) were collected from different biofloc farms. Inductively Coupled Plasma-Mass Spectrometer (ICP-MS) was used to quantify the concentration of metals in fish, fish-feed, and water samples. The concentration (ppb) range of heavy metals is as follows, As (0.47-1.14), Cd(0.09-0.59), Co(0.03-0.33), Cr(0.82-4.60), Cu(0.001-7.01), Hg (0.96-3.37), Ni (1.01 - 10.8), Pb (2.10-4.40), Zn (0.12-73.1), Mn (1.64-18.0), Fe (23.1-261.5) in the collected fishes and As (1.56-1.91), Cd (0.09-0.39), Co (0.25-1.17), Cr (0.001-1.93), Cu (4.96-11.7), Hg (0.90-2.06), Ni (2.19-3.38), Pb (2.93-5.38), Zn (0.002-105.8), Mn (0.01-0.03), Fe (132.3-603.4) in the collected feed sample and also checked the heavy metals concentration in two biofloc fish’s water. The concentrations of selected metals in fish organs and feeds were compared to the safe limits proposed by different international and national institutions. BAF value is found to be highest for Mn and lowest for Co(cobalt). The highest metal shows BAF > 0.5. According to the findings, all fish species have a considerable tendency to acquire metals from their diet. Since THQ>1 was observed, it was clear that the intake of these hazardous metals by consuming these cultured fish does not cause any non-carcinogenic health issues in the human body. Principle component analysis (PCA) analysis showed the metals are higher in the body of fish that are also higher in the tank water and the fish feed
