Method-specific certification of free sugars and starch/glucose in two artificial food materials : BCR-644, BCR-645

This report describes the work performed to prepare two reference materials containing sugars, starch and starch degradation products, and certify them by two methods of analysis. The first method consists in the determination of free sugars (sucrose, fructose and lactose) in food by liquid chromatography. The second method consists in an enzymatic hydrolysis of starch and starch degradation products to glucose, and then in the determination of glucose by liquid chromatography. The materials are powders consisting in mixtures of milk powder, starch, sugars and dextrins. The reference materials can only be used to check the application of the methods A and B described in the Annex C of the report. Certified values were accompanied by an expanded uncertainty according to the requirements laid down in the Guide for the Expression of Uncertainty in Measurement (GUM).