Optimal Complementary Wastewater Mixture for Chlorococcum Sp. Cultivation : Biomass Production, Lipid Accumulation and Wastewater Remediation Studies

This study modelled and optimized biomass and lipid accumulation using mix wastewater for the cultivation of Chlorococcum sp. The simplex lattice mixture design was used to optimize complementary wastewater mixture ratio (25:75, 50:50, 100:0) of dairy (DWW) and paper-pulp (PWW) wastewater. The coefficients of determination ( R 2 ) of 0.91 and 0.98 were obtained for biomass concentration and lipid accumulation models, respectively, demonstrating the fitness of the models for process optimization. The developed optimization model predicted biomass concentration and lipid accumulation of 1.17 g/L and 0.39 g/g, respectively. It suggested 64.69% DWW and 35.31% PWW mixture for biomass concentration, 34.21% DWW and 65.79% PWW for lipid accumulation. Experimental validation generated 0.94 g/L and 0.39 g/g of biomass concentration and lipid accumulation, respectively. High wastewater nutrient removal was observed. These findings demonstrate complementary mixture of DWW and PWW as an excellent media for Chlorococcum cultivation and simultaneous wastewater remediation