Optimization of Cassava Waste from Bioethanol Post-Production through Bioactivity Process Consortium of Saccharomyces cerevisiae, Trichoderma viride and Aspergillus niger

The result of laboratory analysis on bioethanol waste showsnutritional and anti-nutritional content (HCN). Based on it, inorder to prevent environment damage then performed abioconversion of bioethanol waste for base material of sheepforage by fermented previously using consortium ofSaccharomyces cerevisiae, Trichoderma viride, andAspergillus niger to eliminate the waste anti-nutritional contentand increase nutritional content. This research used CompletelyRandomized Design (CRD) factorial pattern with two factorsand three replications. The first factor was inoculum dose (D)and the second factor was variation of tested microorganisms(M). The variables measured were HCN content and nutrientcontent of fermented products, that were water content, proteincontent and crude fiber content by proximate analysis.Furthermore, the data was statistically analyzed by analysis ofvariance (ANOVA), and was there significant differenceanalyzed further by Duncan’s multiple range test of 5% level.Nutritional compositions of bioethanol waste from cassava thathad been fermented by consortium of Saccharomycescerevisiae, Trichoderma viride, and Aspergillus nigerexperienced changes compared to before fermentation. Theresults showed an increase in protein level, while fiber content,water content, and HCN content were decrease. Bioethanolwaste from cassava fermented by Aspergillus niger andSaccharomyces cerevisiae (k3) with 2% inoculum dose had thehighest protein content increase from 11.79% to 25.41% andhad the lowest crude fiber content decrease from 16.4% to12.84 %. The highest HCN decrease obtained from thefermentation of consortium Aspergillus niger and Trichodermaviride.