Optimization of Alkaline Flocculation for Harvesting of <i>Scenedesmus quadricauda</i> #507 and <i>Chaetoceros muelleri </i>#862

A response surface methodology (RSM) was used to evaluate the effects of pH and microalgal biomass concentration (BC) on alkaline flocculating activity for harvesting one freshwater green algae <i>Scenedesmus quadricauda</i> #507 and one marine diatom <i>Chaetoceros</i> <i>muelleri</i> #862. The pH value and BC were in range of 9.0–12.0 and 0.20–2.30 g/L, respectively. Very high regression coefficient between the variables and the response indicates excellent evaluation of experimental data by second-order regressions. Optimum conditions for flocculating activity were estimated as follows: (i) pH 11.6, BC 0.54 g/L for strain #507 and (ii) pH 11.5, BC 0.42 g/L for strain #862. The maximum flocculating activity was around 94.7% and 100%, respectively. Furthermore, the addition of synthetic ocean water (SOW) to the freshwater #507 culture can increase the flocculating activity from 82.13%–88.79% in low algae concentration (0.52 g/L) and 82.92%–95.60% in high concentration (2.66 g/L). <b> </b>