The certification of reference materials of dry-mixed maize powder with different mass fractions of 1507 maize : certified reference materials ERM-BF418 (ERM-BF418a, ERM-BF418b, ERM-BF418c, ERM-BF418d)

This report describes the preparation and certification of dry-mixed maize powder CRMs with different mass fractions of genetically modified (GM) 1507 maize powder. The CRMs were processed in 2004 and certified in 2005 by the European Commission, Directorate General Joint Research Centre, the Institute for Reference Materials and Measurements (IRMM) in Geel, Belgium. The CRMs are intended for the quality control and calibration of methods for the detection of genetically modified organisms. The 1507 mass fractions of ERM-BF418 were verified with the help of DNA-based detection methods. Seeds of non-modified maize and 1507 maize both supplied by Pioneer Hi-Bred International (Johnston, IA, USA) were rinsed with demineralised water, drained and dried at 30° C in order to minimise dust contamination from other crops. After a two step grinding process, the materials were prepared by turbula-mixing and dry-mixing of non-modified maize powder and 1507 maize powder.