In this study, it was aimed to determine the changes in some physicochemical properties of different media used in seedling cultivation and tomato (Solanum lycopersicon cv.) seedling quality parameters. In the study, growing media were created from eight different mixtures of peat, diatomite, zeolite and vermicompost. KAYRA F1 tomato variety was used as seedling. At the end of the 45-day trial period; the amount of water content of medium in different tension values (pF0, pF1.30, pF1.66, pF1.77, pF1.90, pF2.00, pF2.54 and pF4.2), pH and EC values, volume weight and cation exchange capacity (CEC) was determined. In tomato, seed germination percentage (%), seedling length (cm), root length (cm), stem diameter (mm), seedling fresh and dry weight (g), fresh and dry root weight (g) and chlorophyll content of seedling was examined. The highest seedling fresh weight, seedling dry weight, root fresh weight and root dry weight were in M8, and the best seedling height was in M4 medium. The change in seedling root length was found to be insignificant. The best germination percentage was determined in M1, the best chlorophyll content was determined in M6 medium. The highest available water amount was obtained from the M1 medium, while the lowest was obtained from the M6 medium. In the study, it is predicted that the best growing medium for tomato seedling yield and quality parameters is M8, and positive results can be obtained in terms of quality seedling cultivation in M6, M5 and M4 medium