Event-specific method for the quantification of cotton line GHB614 using real-time PCR : validation report and protocol
The JRC as Community Reference Laboratory for GM Food and Feed (CRL-GMFF), established by Regulation (EC) No 1829/2003, in collaboration with the European Network of GMO Laboratories (ENGL), has carried out a collaborative study to assess the performance of a quantitative event-specific method to detect and quantify the GHB614 transformation event in cotton DNA (unique identifier BCS-GH002-5). The collaborative trial was conducted according to internationally accepted guidelines. In accordance with Regulation (EC) No 1829/2003 of 22 September 2003 on genetically modified food and feed and with Regulation (EC) No 641/2004 of 6 April 2004 on detailed rules for the implementation of Regulation (EC) No 1829/2003, Bayer CropScience provided the detection method and the samples (genomic DNA from cotton plants containing the transformation event GHB614 and from conventional cotton plants). The JRC prepared the validation samples (calibration samples and blind samples at unknown GM percentage [DNA/DNA]). The collaborative trial involved twelve laboratories from ten European countries. The results of the international collaborative trial met the ENGL performance requirements. The method is therefore considered applicable to the control samples provided, in accordance with the requirements of Annex I-2.C.2 to Commission Regulation (EC) No 641/2004.
Alternative title: | Event-specific method for the quantification of cotton line GHB614 using real-time PCR Event-specific method for the quantification of cotton line GHB614 using real-time PCR Cotton seeds sampling and DNA extraction |
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Year of publication: |
2008
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Other Persons: | Savini, C. (contributor) ; Bogni, A. (contributor) ; Mazzara, M. (contributor) |
Institutions: | European Commission / Joint Research Centre (issuing body) |
Publisher: |
Luxembourg : Publications Office |
Saved in:
freely available
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