Rapid Quantification Of Sulfonamides Using Ag Nanoislands/Fluorinated Si Nanopillars Assisted Laser Desorption Ionization Mass Spectrometry

In recent years, the abuse of antibiotics has led to adverse effects on water environment and food safety, and to monitor the antibiotic content in water and foodstuffs is imperative. In this work, Ag nanoislands/fluorinated Si nanopillars substrate is prepared for sensitive and rapid detection of sulfonamides using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). The large surface area and roughness of the Si nanopillars are important characteristics of the substrates, which can not only efficiently absorb UV laser, but also induce the formation of isolate Ag nanoislands. The SALDI-MS performance is enhanced on the Ag nanoislands/fluorinated Si nanopillars than on Ag nanoislands/Si nanopillars, which can be attributed to the higher homogeneity of Ag nanoislands on the fluorinated Si nanopillars with lower surface energy. Profited from the synergistic effect of Ag nanoislands and Si nanopillars, the heterostructures present high detection reproducibility and sensitivity. Furthermore, the coefficients of determination (> 0.99) are satisfactory when quantifying four sulfonamides without internal standards, which shows that the substrate is applicable for rapid quantification of sulfonamides. Ultimately, the sulfacetamides spiked in milk and lake water can be rapidly identified and quantified. The prepared substrate presents a considerable potential for food and environment monitoring