Report on the method performance study of a method to determine phthalates in wine : determination of ten phthalates in wine by gas chromatography mass spectrometry (gc-ms)

The Institute for Reference Materials and Measurements (IRMM) organised in close collaboration with the International Organisation of Vine and Wine (OIV) a collaborative study to assess the performance of an analytical procedure for the determination of ten phthalates in wine by gas chromatography - mass spectrometry (GC-MS). The tested analytical procedure (OIV-MA-AS323-10:2013) was endorsed in June 2013 by the General Assembly of OIV and taken up in the Compendium of International Methods of Analysis of Wines and Must. The design of the method performance study complied with provisions given in ISO 5725-2. It comprised the analysis of six different wine samples as blind duplicates. Test samples consisted of red wine, white wine (both supplied by the SCL Laboratoire de Bordeaux), and sweet wine, which was acquired in Belgium at a local store. The wines were spiked at IRMM with the analytes to levels suggested by OIV, bottled into ampoules, and dispatched to the participants of the validation study. The participants of the study were identified by OIV. They comprised laboratories from Europe, Asia, South America and Australia. The evaluation of the reported results was performed according to ISO 5725-2 and ISO 5725-4. It revealed acceptable precision of the analytical method for the determination of the analytes in most test materials. Relative standard deviations for reproducibility were mostly within the range of 9 % to 71 %. However, significant method related bias was observed for the determination of several analytes. The application of the analytical method for the determination of phthalates in contaminated wine samples could cause underestimations of the real content by up to about 60 %.