The certification of reference materials of dry-mixed soya powder with different mass fractions of Roundup ReadyTM Soya IRMM-410S : IRMM-410S

This report describes the preparation and certification of dry-mixed soya powder CRMs (IRMM-410S-0) with different mass fractions of genetically modified Roundup Ready TM soya powder (Certified Reference Materials IRMM-410S-0 IRMM-410S-1 IRMM-410S-2, IRMM-410S-3, IRMM-410S-4 and , IRMM-410S-5). The CRMs were produced in 2001 by the Joint Research Centre of the European Commission, the Institute for Reference Materials and Measurements (IRMM) in Geel, Belgium. The CRMs are intended for the calibration of methods for the detection of genetically modified food. The Roundup Ready TM concentration of IRMM-410S was verified with the help of DNA-based and protein based detection methods. The CRMs are available in the form of glass bottles containing 1 g of soya powder packed under argon atmosphere. Seeds of non-modified soya (line a1900) and Roundup Ready TM (event 40-3-2, line AG5602 RR) were rinsed with demineralised water, additionally drained and dried at 30 °C in order to minimise dust contamination from other crops. The materials were prepared by quantitative Turbula and dry-mixing of non-modified soya powder and 100% Roundup Ready TM GMO soya powder. It is recommended to use samplings not smaller than 100 mg. Bottles should be stored dry and cool (+4 °C) and kept in the dark.