Verification of performance of Ms8 and Rf3 event-specific methods on the hybrid Ms8xRf3 using real-time PCR : validation report
The JRC as Community Reference Laboratory for GM Food and Feed (CRL-GMFF) (see Regulation EC No 1829/2003), has carried out an in-house verification study to assess the performance of two quantitative, event-specific methods for use with the hybrid oilseed rape line (unique identifier ACS-BN 5-8xACS-BNøø3-6) which combines both the Ms8 and Rf3 transformation events. Both methods have been previously fully validated individually on parental lines, to detect and quantify each insert on extracted DNA. The study was conducted according to internationally accepted guidelines (1,2). In accordance with Regulation (EC) No 1829/2003 of 22 September 2003 on genetically modified food and feed and with Regulation (EC) No 641/2004 of 6 April 2004 on detailed rules for the implementation of Regulation (EC) No 1829/2003, Bayer CropScience provided the detection methods and the samples (genomic DNA extracted from the wild-type and 100% event Ms8xRf3 hybrid oilseed rape). The JRC prepared the in-house validation samples (calibration samples and blind samples at unknown GM percentage). The results of the in-house verification study were evaluated with reference to ENGL method performance requirements (http://gmo-crl.jrc.it/doc/Method%20requirements.pdf) and to the validation results for the individual parental lines (http://gmo-crl.jrc.it/statusofdoss.htm). The results of CRL-GMFF in-house verification studies are publicly available at http://gmo-crl.jrc.it/.
Alternative title: | Event-specific method for the quantification of oilseed rape line Ms8 using real-time PCR Event-specific method for the quantification of oilseed rape line Rf3 using real-time PCR |
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Year of publication: |
2007
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Other Persons: | Mazzara, M. (contributor) ; Grazioli, E. (contributor) ; Savini, C. (contributor) |
Institutions: | European Commission / Joint Research Centre (issuing body) |
Publisher: |
Luxembourg : Publications Office |
Saved in:
freely available
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