Verification of performances of NK603 and MON 810 event-specific methods on the hybrid NK603 x MON 810 using real-time PCR : validation report

The JRC as Community Reference Laboratory (CRL) for the GM Food and Feed (see Regulation EC 1829/2003), has carried out an in-house verification study to assess the performance of two quantitative, event-specific methods, previously validated on the parental lines, to detect and quantify the MON 810 and the NK603 transformation events on seeds from the hybrid maize line combining the two thereof traits (unique identifiers MON-00603-6 x MON-00810-6). The study was conducted according to internationally accepted guidelines. Monsanto Company provided the method-specific samples (seeds NK603 x MON 810 and null) whereas the JRC prepared the verification samples (calibration samples and blind samples at unknown GM percentage).